If the target protein is of low molecular weight a high percentage gel should be chosen in order to maximize protein resolution. Selecting the correct gel for the target protein can be a crucial step in optimizing detection and quantification. In general Bio-Rad recommends its Criterion™ Tris-tricine precast gels for small proteins and its Criterion XT Tris-acetate precast gels for separating higher molecular weight proteins (see section below for more details). Knowing the size of the target protein will indicate the gel that should be chosen and the conditions for transfer. One disadvantage of protein precipitation is that proteins might denature, making the pellet difficult to re-solubilize. After centrifugation of the precipitated protein to a pellet, the supernatant is removed and the protein pellet is re-dissolved in sample buffer. In addition to a nuclear protein extraction Bio-Rad has protein extraction kits for membrane bound proteins as well as cytosolic signaling proteins.Īnother strategy for increasing specific protein detection is to add a compound such as acetone that causes protein to precipitate. An anti-lamin A/C antibody ( VMA00200) can be used as a marker to differentiate nuclear from cytoplasmic samples. For recovery of nuclear proteins, Bio-Rad offers the ReadyPrep™ Protein Extraction Kit (Cytoplasmic/Nuclear). If the target protein is primarily expressed in a single cellular compartment (membrane or nucleus) then recovery of the proteins from the individual component will increase the chances of quantifiable detection. Recovery of protein from individual cellular compartments Reduce Cu 2+ to Cu + which binds to protein Under acidic conditions Coomassie Brilliant Blue G-250 binding to protein Peptide bonds react with copper under alkaline conditions to produce Cu + They are all colorimetric assays and can utilize known concentrations of Bovine Serum Albumin (BSA) to construct a standard curve against which samples can be evaluated. There are three primary methods for determining the concentration of recovered protein. Approximate Protein Recovery (based on HeLa cells, the number of cells will vary according to cell type).ĭetermining concentration of recovered protein Typical loading amounts of protein added to each well range from 10 µg (~330,000 cells) to 50 µg (~1,650,000 cells). HeLa cells contain approximately 300 pg per cell. Protein recovery can vary according to the cell type and recovery method. However, please note that using an insufficient amount of lysis buffer may lead to an incomplete lysis of the sample. This will allow the addition of increased amounts of protein per well furthering chances of detecting the low abundant protein. When lysing the cell or tissue, use as little lysis buffer as possible in order to concentrate samples as much as possible. Use of the correct lysis buffer will improve the detection of the low abundant target protein. Non-ionic detergents induce less protein denaturation so if their use is often recommended with primary antibodies raised against native proteins. If further denaturing is required a reducing agent (DTT or Beta mercaptoethanol) may be used to disrupt S-S bonds. As such their use with antibodies raised against denatured forms or synthetic peptides of the target protein is often recommended. Ionic detergents are considered harsher than the non-ionic detergents because of their ability to interfere with protein structure. The best known of the ionic detergents is sodium dodecyl sulfate (SDS) while non-ionic detergents include Tween, Triton X and NP-40. The primary active agent in lysis buffer is the detergent which can be ionic or non-ionic. Lysis is the process in which the cell membrane is broken and the contents of the cell are re-suspended in a soluble form. There are several steps for maximising the recovery of your target protein from the sample. Initial improvements in the detection and quantification of low abundant proteins can be made at the sample preparation stage. As such detection of proteins with low expression levels is a common problem for researchers. To help you optimize both the detection and quantification of such proteins and overall quality of the final western blot image, we have put together useful tips for the following steps: The technique of western blotting illuminates molecular events including protein expression, protein localisation, protein-protein interactions or post translational modifications (PTMs). These molecular events are often very subtle. For example within an individual signaling pathway only a certain percentage of the molecules may have undergone a PTM. Enhance detection and quantification of low abundance protein
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Each one of you has contributed to our collective success, and tonight, we shine a spotlight on those who have gone above and beyond, truly embodying the spirit of excellence. So, without further ado, let us embark on this memorable journey together.Įmcee: As we gather here tonight, we reflect upon the incredible talent and dedication that exists within our organization. It’s a night filled with anticipation, inspiration, and a genuine sense of pride. Tonight, we gather here to celebrate and recognize the remarkable achievements of our outstanding individuals and teams. Emcee Script Example #1Įmcee: Ladies and gentlemen, distinguished guests, and esteemed colleagues, a warm welcome to the Annual Excellence Awards Ceremony! I am honored to be your emcee for this prestigious event. Be prepared to think on your feet, improvising when necessary, and seamlessly adjusting the script to accommodate unforeseen circumstances or time constraints. Embrace unexpected changes or last-minute adjustments gracefully. While a well-prepared script is essential, it’s equally important to remain flexible and adaptable during the event. Rehearse your script multiple times to build confidence and ensure a smooth and natural delivery. Familiarize yourself with the flow, timing, and emphasis on key points. Practice and Rehearsal: Once your script is complete, practice delivering it aloud. Similarly, create a powerful closing that leaves a lasting impression and reinforces the event’s key message. Utilize a mix of short, punchy sentences and longer, descriptive sentences to create a dynamic rhythm in your delivery.Ĭaptivating Opening and Closing: Craft attention-grabbing opening remarks that immediately capture the audience’s attention. Varied Sentence Structure: Vary sentence lengths and structures to maintain the audience’s interest. Add appropriate humor, anecdotes, or personal insights, but be mindful of the event’s tone and audience preferences. Injecting Personality: Infuse your own personality and style into the script to create a unique and memorable experience. Strike a balance between professionalism and approachability. Avoid complex jargon or technical terms that may alienate the audience. Consider the following tips:Ĭlear and Concise Language: Use simple and concise language to ensure clarity and easy comprehension. The writing style and tone of an emcee script significantly impact the audience’s engagement. Summarize the key highlights of the event and leave the audience with a lasting impression or call to action. Express gratitude to participants, sponsors, and the audience. Ensure these interactions are well-planned and align with the event’s objectives.Ĭlosing Remarks: Conclude the event with a memorable closing. This can include asking questions, conducting polls, or encouraging participation in activities related to the event. Interactions and Engagement: Inject moments of audience engagement to keep their interest alive. Maintain a respectful and enthusiastic tone throughout. Tailor the introduction to match the tone and purpose of the event, highlighting the relevance of their contribution. Introducing Speakers or Performers: When introducing speakers or performers, provide a brief but impactful biography emphasizing their expertise or achievements. Use transition phrases, such as “Now, moving on to our next segment,” to guide the audience seamlessly. Prepare concise and clear transitions that bridge one part of the event to the next. Transitions: Smooth transitions between segments or speakers are crucial for maintaining the event’s momentum. Briefly outline the agenda for the event, highlighting key segments or speakers to generate anticipation. Share a relevant anecdote, quote, or interesting fact to captivate the audience. Opening Remarks: Set the tone for the event by delivering compelling opening remarks. Provide a brief overview of the event and express enthusiasm for being part of the occasion. Introduce yourself and establish your role as the emcee. Introduction: Start with a warm welcome that captures the audience’s attention. Structuring the Emcee ScriptĪ well-structured emcee script is essential for maintaining a smooth flow and keeping the audience engaged. This will enable you to connect with them on a personal level, ensuring a more engaging and inclusive experience. Additionally, consider the demographics and interests of the audience. Is it a formal occasion or a casual gathering? Is it educational, celebratory, or a combination of both? Understanding these aspects will help you tailor your script accordingly. Consider the purpose, theme, and tone of the event. The first step in writing an emcee script is to thoroughly understand the nature of the event and the expectations of the audience. Final Thoughts Understanding the Event and Audience. I don’t know why the Anti-Villain League only decided to act on this pharmacist now when he’s been active all summer, but apparently this is big enough of a threat to send you in.” “Apparently, some pharmacist named Hanz Boobenplatz or however you pronounce it has been perfecting hammerspace technology, and has built all sorts of contraptions. “The Anti-Villain League’s been noticing some, uh… fishy activity in Danville,” Nefario briefed his superior. Nodding his head while chewing on his hotdog, Gru beckoned Nefario to continue speaking. “Anyway, now that you’re near your destination, let’s go over your mission again.”Īs he continued driving and eating his Slushy Dawg, Gru sighed internally, remembering having heard the overwrought briefing by the superior of the Anti-Villain League a few hours earlier. “Slushy Dawg? Isn’t that the fast food restaurant that never gets any better?” Nefario inquired. I couldn’t help it I forgot my extra snacks.” “I did stop by to buy a hotdog from Slushy Dawg, though. Gru nodded, fishing out a hotdog sandwich of sorts with his free hand. “Gru,” began Gru’s most loyal subordinate, Dr. The large man’s phone rang, and he quickly responded, putting the device on loudspeaker. Who was driving that car, you might ask? Well, the man driving the large vehicle was none other than Felonius Gru, formerly the most despicable villain in the world, now an agent of the world-renowned Anti-Villain League. However, neither the motorists nor the people in the sidewalk paid it too much attention, as judging from the looks in their faces, they had seen better or cooler things in this town. Of course, one of the few things that would have stood out was the large, oddly-constructed car that was currently driving through the streets towards its destination. People were walking the sidewalks and entering buildings to go about their daily business, and vehicles of all assortments were driving through the streets of the city, falling in line with regular traffic rules. Today was a pretty normal day in the city of Danville. PRELUDE HERE: Prelude: Despicable-inator! TheMHFan: It’s time for a DEATH BATTLE!!! Bringer of Darkness: Alright, the combatants are set, and we’ve run the data through all possibilities! Some population estimates for territories are from the United Nations Commission on Population and Development. states by population density List of U.S. Separate statistics are maintained for the five permanently inhabited territories of the United States: Puerto Rico, Guam, the U.S. Population data are for the year 2023 and area data are for the year 2010. states by historical population (tables of state populations since 1790) List of U.S. The states and territories included in the United States Census Bureau s statistics for the United States population, ethnicity, religion, and most other categories include the 50 states and Washington, D.C. Data are from the US Census unless otherwise specified. Population density is defined as the population divided by land area. States and territories by population density On the other hand, it is over 8 times higher than that of Canada and over 9 times higher than that of Australia. is 1/15 that of South Korea and 1/12 that of India and the Netherlands. For comparison, the population density of the U.S. There are large, sparsely populated areas in parts of the US, like the east-to-west stretch extending from the outskirts of Seattle all the way to Minneapolis, or the north-to-south portion from northern Montana to southern New Mexico, excluding Denver. The population density of the United States is lower than that of many other countries because of the United States' large land area. It also includes a sortable table of density by states, territories, divisions, and regions by population rank and land area, and a sortable table for density by states, divisions, regions, and territories in square miles and square kilometers. Closely followed by China, Macao SAR with a population. The country with the highest population density was Monaco with a density of 24779.9 people per square kilometer. This is a list of the 50 states, the 5 territories, and the District of Columbia by population density, population size, and land area. In the year 2020, the world had a population density of 60.1 (people/km²). Map of states shaded by population density (2020) This is a list of the 50 states, the 5 territories, and the District of Columbia by population density, population size, and land area. Urban and rural population in the U.S.Map of states shaded by population density (2020) Population density map of what is now the United States (1492) It isn’t easy to evaluate populations in the 15th century in America. Population density in California 1960-2018 Population density in New Jersey 1960-2018 metro areas with the highest concentration of high-income households 2011 Population density in Tennessee 1960-2017 The below image explains the population density of the state and the 50 states of the United States show different increases rate of population in each state. The degree of urbanization has also increased, and well over half of the population lives in urban centers. While the United States is not very densely populated compared to other countries, its population density has increased significantly over the past few decades. However, much of the country is uninhabited, so cities in Russia are much more densely populated than the rest of the country. While this can be an interesting measure of how many people live in a country and how large the country is, it does not account for the degree of urbanization, or the share of people who live in urban centers.įor example, Russia is the largest country in the world and has a comparatively low population, so its population density is very low. Simply put, population density is the population of a country divided by the area of the country. As a whole, there were about 94 residents per square mile in the U.S., and Alaska was the state with the lowest population density, with 1.28 residents per square mile. had the highest population density in the United States, with 10,984.43 people per square mile. Don’t forget to check the spelling and grammar-it’s essential for a professional-looking newspaper! Step 4: Insert Images and CaptionsĮnhance your newspaper with relevant images and captions.Īrticles with images are more engaging and help break up blocks of text. Then, start typing your articles, or paste text you’ve written elsewhere. Use the “Home” tab to choose a bold and readable font style and size for your headlines. Insert headlines and type in your articles using Word’s text editing tools.Įvery newspaper needs attention-grabbing headlines. Keep in mind that you can adjust the width and spacing of columns to best suit your content. To adjust columns in Word, go to the “Layout” tab and click on “Columns.” Choose the number of columns you want and apply them to your document. Most newspapers use a multi-column format for a traditional look and to fit more content onto each page. Step 2: Adjust the ColumnsĬustomize the template’s column layout to fit your newspaper’s style. Once you’ve selected a template, it will open as a new document, and you’re ready for the next step. Microsoft Word offers a variety of templates, and you can find them by searching for “newspaper” in the template search bar. Templates are a great starting point because they come with a predefined layout that you can customize. Start by opening Microsoft Word and selecting a newspaper template. By following these steps, we’ll transform a blank Word document into a multi-column newspaper with headlines, articles, and images. So grab your journalist’s hat, and let’s dive in! Step by Step Tutorial to Create a Newspaper in Microsoft Wordīefore we start, let’s define what we will accomplish. In this article, we’ll guide you through the steps to create your newspaper in Word, from choosing the right template to adding finishing touches. With a little bit of patience and some creativity, anyone can produce a newspaper that looks professional and engaging. It’s also a great way to develop your design and layout skills. Making a newspaper in Microsoft Word is a fun and rewarding project. Whether you’re a teacher looking for a classroom project, a small business owner wanting to connect with customers, or a community member eager to share local stories, creating a newspaper could be just what you need. A newspaper can be a fantastic platform for sharing news, expressing opinions, and showcasing creativity. Have you ever wanted to create your newspaper but didn’t know where to start? Well, you’re in luck because Microsoft Word provides all the tools you need to design and publish your very own paper. Intended specifically for managing collections in archives and records officesĪ cross-platform runtime environment for building rich Internet applications using Adobe Flash, Adobe Flex, HTML, or Ajax, that can be deployed as a desktop applicationĪleph provides academic, research, and national libraries with the efficient, user-friendly tools and workflow support Used for the ECDL Driving Licence examinations. Reference management software package, used to manage bibliographies and references when writing essays and articles.ħ-Zip is a file archiver with a high compression ratio.ĭesigned to view, create, manipulate and manage files in Adobe's Portable Document Format (PDF). See here for changes coming to ArcGIS - AnywhereĪrcmap city engine, student home use, data loaderĭesigned to view, create, manipulate and manage files in Adobe's Portable Document Format AnywhereĪ fully-interactive visualization and analysis of crystal and molecular Anywhere MATLAB s a high-level language and interactive environment that enables you to perform computationally intensive tasks faster than with traditional programming languages such as C, C++, and Fortran. Ibm, amos, Sample, Power, pasw, Student home use # A A B B C C D D E E F F G G H H I I J J K K L L M M N N O O P P Q Q R R S S T T U U V V W W X X Y Y Z Z NameĪ statistical Package, designed for analysing data. Open your IDE, making sure to use version 2021.1 or later. If you've configured your JetBrains product to use a proxy to connect to the internet, make sure that the URLs mentioned in this article are reachable via your proxy. If you're using a proxy, is License Vault reachable through it? If in doubt about which credentials you should use, contact your administrator. To obtain a license from License Vault, you'll need to log in through the authentication provider configured by your administrator. Is your product version 2021.1 or higher?Īctivation via License Vault is not supported in earlier versions of JetBrains products. If you restart your product while disconnected, you'll immediately lose access to your license and won't be able to use the product until you reconnect to the internet. After 48 hours, you'll have to reconnect to the internet to continue using the product. You can work for up to 48 hours without an internet connection, as long as you don't restart the product. Are you connected to the internet?Īn internet connection is required both to obtain a license from License Vault and to continue using it. If that doesn't work, contact your administrator to get the link. If you don't have the link, you can try using the automatic server discovery at step 4 of the activation instructions below. Before activationīefore you activate your JetBrains product, go through the following checklist to make sure you have everything necessary to successfully obtain a license. This section walks you through the process of obtaining a product license from License Vault. Here, we will use an existing vector asset, the USGS Watershed Boundaries - HUC12 We do this in the Classify Imagery Module. Manually draw points, lines, and polygons using the geometry tools in the code editor.(Browse the vector dataset catalog here). Use an existing vector dataset in GEE.If you have the choice, I’d use an asset. GEE only recently added the Asset option, so you may see folks still using fusion tables in the forums, etc. Each fusion table has a unique Id (File > About this table) that can be used to load it into GEE. Import an existing Google Fusion Table, or create your own fusion table from a KML in WGS84.We use an asset vector file in the Accessing Satellite Imagery module. You can create subfolders and set sharing permissions on these as needed. Upload a shapefile directly to your personal Asset folder in the top left panel.There are four ways to obtain vector data in GEE: The easiest way to filter for an irregular location without having to identify the paths and rows of the satellite image tiles is to use a vector polygon. We’ll work on making a composite satellite image for a US watershed. Read more about filters on the Developer’s Guide. There are shortcut filters for those commonly used (imageCollection.filterDate(), imageCollection.filterBounds()…), but most filter in the ee.Filter() section of the Docs tab can be used. In order to generate images that cover large spatial areas and to fill in image gaps due to clouds, etc, we can load a full ImageCollection but filter the collection to return only the time periods or spatial locations that are of interest. More information on Image Collections can be found here in the GEE Developer’s Guide. You can also create image collections from individual images or merge existing collections. Each data source available on GEE has it’s own Image Collection and ID (for example, the Landsat 5 SR collection, or the GRIDMET meteorological data collection). Image CollectionsĪ stack or time series of images are called Image Collections. Here, we will leverage GEE to create a composite satellite image representing the peak growing season for a watershed of interest. Google Earth Engine (GEE) is particularly well suited to these tasks.Ī static version of the code can be accessed here: * Exercise: Basic GEE Workflow However, cloudy skies during satellite overpass and other acquisition anomalies make certain scenes or pixels unusable.Ĥ55 Landsat scenes cover the United States:įor most regional-scale applications, you will need to combine multiple satellite images to fully cover your spatial extent and fill in missing data caused by clouds, etc. The edges of each path overlap, providing increased temporal frequency in these areas. Each scene is currently imaged every 16 days by Landsat 8, and every 16 days by Landsat 7 (approximately 45 times each year). Global Landsat data is broken up in ~180 km 2 scenes, with unique path/row identifiers. Most satellite products are broken up into tiles for distribution. Overview: Satellite Imagery at Regional Scales Understand how to map functions across image collections Perform basic image manipulation, mosaicking, and visualization Learn how to access images based on filter criteria We also recommend that you have the latest graphics drivers (WDDM) installed on your Windows PC. To determine if this codec is already installed, open the Microsoft Store app, and search for it there- AV1 Video Extension. To determine if this codec is already installed, open the Microsoft Store app, and search for it there- VP9 Video Extensions.ĪV1. You’ll need this codec to play HDR video on YouTube. If this codec isn’t installed, you can download it from the Microsoft Store - HEVC Video Extensions. To determine if this codec is installed, open Settings, select Apps > Apps & features, then search for video extension. You’ll need this codec and a premium subscription to play HDR video on Netflix. Here are the common codecs that are required to play HDR video: Some codecs might already be installed on your PC. Devices using modern processors support this capability. Your Windows 11 device needs to have an integrated graphics card that supports PlayReady hardware digital rights management (for protected HDR content), and it must have the required codecs installed for 10-bit video decoding. To find out if your display is HDR certified, visit the device manufacturer’s website. If you go to Settings > System > Display > Advanced display and your display doesn’t show as certified (it says Not found next to HDR certification), it might be because it doesn’t have an HDR certification, or the display manufacturer hasn’t published HDR certification information for your display. Look for displays that meet the DisplayHDR 500 or higher tiers. Look for the latest DisplayHDR certification-version 1.1 certified displays. Here are some tips for getting the best experience: VESA DisplayHDR - DisplayHDR certification has multiple tiers-a higher number indicates a higher tier. Here are the different HDR certifications to look for when buying an HDR-capable display or Windows 11 PC: Whether you have a built-in or external display, we recommend you look for displays that are certified for HDR. To find the specifications for a specific PC or external display, visit the device manufacturer’s website. The HDR display or TV must support HDR10, DisplayPort 1.4, HDMI 2.0 or higher, USB-C, or Thunderbolt. To find the specifications for a specific laptop or tablet, visit the device manufacturer’s websiteĮxternal display. The built-in display needs to have a resolution of 1080p or more, and a recommended max brightness of 300 nits or more. Your display can be a built-in display, like the kind on a laptop, tablet, or 2-in-1 PC, or it might be an external display that’s connected to your PC.īuilt-in displays. To play HDR video in Windows 11, your display, PC, and graphics card must meet certain requirements. The palette is a little bit on the garish side (think Quake I: lots of browns and blues, here it's gray/red/blue and the other colors are used sparingly). The game play is really repetitive: Kill, Kill, Kill, down some potions, grab some loot, Kill some more, go back to Tristram and sell the stuff you've looted, buy some new gear, go back to the dungeon(s) to kick some more baddies in the ass, rinse and repeat dozens of times until you get to Diablo and kick his ass too! The gory backgrounds (downright nasty sometimes: check out the impaled guys in the later stages!) and dark ambiance (almost too dark: you'll need to crank the gamma a bit here) really suck you in, the simple but engaging game play does the rest as the hours will literally fly as you go deeper underground and butcher tons of baddies in order to kick Diablo's ass. The combination of a gritty, dark visual presentation along with a terrific understated soundtrack and immense re playability makes for an addictive hack and slash. Total Entertainment Network (TEN) multiplay platform.Games with randomly generated environments.
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